Composite

Part:BBa_K4062035

Designed by: Benjamin Bateman   Group: iGEM21_WLC-Milwaukee   (2021-10-21)


lacI with lac operon regulatory components, lacZ RBS mutation

This part contains a mutated lacZ RBS. The RBS was changed from AGGAAA to CCCTTT. It also includes the lacI promoter, lacI ORF, CAP site, lacP, and lacO.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None